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Epidemiological studies suggest that a venereally transmitted infectious agent such as HPV`s could play a important role in the etiology of human cervical carcinoma. Over 60 different types of HPV have been identified. However, only certain types are commonly found in female genital genital lesions, including HPV-6 and HPV-11, associated with condylomata accuminata, and HPV-16 and HPV-18, associated with cervicaldysplasia and carcinoma. Various methods have been successfully used for the detection of HPV in genital lesions, including antigenic analysis, Southern blot hybridzation, dot blot hybridization and in situ hybridization. HOwever these methods are cumbersome and often lack sensitivity. A very sensitive polymerase chain reaction procedures capable of amplifying specific DNA sequeces by up to millionfold was recently developed for detection of HPV DNA. In this study, we performed the PCR procedures to detect HPV-16,-18 in cervical scrape speciments obtained from cervical intraepithelial neoplasia (CIN; 22 cases) and invasive squamous cell carcinoma (32 cases) and normal cervix (25 cases). And we compared the prevalence of HPV`s with the histologic diagnosis and findings of Pap smears. The results were as follow; 1. The detection rate of HPV-16 DNA in normal cervix, dysplasia, CIS and invasive squamous cell carcinoma were 44.0%(11/25), 62.5%(10/16), 83.3%(5/6), 96.9%(31/32), respectively. 2. The detection rate of HPV-18 DNA in normal cervix, dysplasia, CIS and invasive squamous cell carcinoma were 4.0%(1/25), 18.8%(3/16),0%(0/6), 15.6%(5/32) respectively. 3. The co-infection rate of HPV-16 and -18 in normal cervix, kysplasia, CIS and invasive squamous cell carcinoma were 4.0%(1/25), 12.5%(2/16), 0%(0/6), 15.6%(5/32) respectively. 4. The results of comparison of comparison between the prevalence of HPV`s and the findings of Pap smears were as follows;. HPV DNA Class I Class II Class III ClassⅣ ClassⅤ No.(%) No.(%) No.(%) No.(%) No.(%) Type 16 9/21(42.9) 4/6(66.7) 8/14(57.1) 8/9(88.9) 28/29(96.5) Type 18 1/21(4.8) 0/6(0.0) 3/14(21.4) 0/9(0.0) 5/29(17.3) In cervical scrape specimens, detection of HPV DNA using the PCR is considered to be very rapid, easy and sensitive method for detection of HPV DNA and also helpful to screen the women with a high risk of cervical cancer. And HPV maybe a important factor in the development of cervical cancer, but the roles of HPV-16 and HPV-18 in the development of cervical cancer maybe different each other.
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